Then add a washer and nut as spacers to allow air to reach the back of the fan. Push the screws thru the control box and the cooler. Drill small holes (5/32”) for the screws and a larger hole (1/2”) for the wires from the fan, heater, and the temperature probe (see Figure 2). Mark the four fan mounting holes and a center hole. Start by deciding where you want the heater and control unit and hold the fan against the outside of the cooler.
Be careful to get it where you want it the first time (voice of sad experience).Ī set of four #6 screws start inside the control box, penetrate the cooler wall, go thru the fan’s mounting holes, and thru the holes drilled in the can. The adhesive on the heater pad is very strong and temperature resistant. Then, mount the heater pad by sticking it to the inside of can. Next, cut the top leaving tabs that can be bent inwards, drilled and used as mounting points (see Figure 3). Remove the ends, then use shears to cut the can down to about three inches. MakerFocus 4pcs 3D Printer Fan 12V, 40mm 12 Volt Fan Icstation Flexible Polyimide Heater Plate Adhesive PI Heating Temperature Controller Module with Case, XH W1209 Display I have had good success with Igloo brand coolers That way I can be reasonably sure that growth rate differences observed in different incubators is due to temperature.Īlso, if I had to run a 70° test, then later a 75° test, then later an 80° test I would have to be more patient person.
I make up a large batch of plates or flasks or boxes and randomly assign them to different temperature incubators. Then run everything in parallel with multiple replicates. So if you want to measure growth rates in response to temperature you also want to minimize growth rate effects caused by different isolates and culture media.Ī simple way to do this starts with a large batch of homogenous culture medium and a single subdivided mycelial colony. Growth rate is known to vary from one batch of growing media to the next, and different mycelial isolates can grow at different rates. Uncontrolled sources of error are to be minimized. Incubators from scientific supply houses usually have less precision, much higher cost, and are often not for sale to individual experimenters. The device detailed here will swing only ± 0.5° F. Now consider growing your cultures at 70°, 75°, 80°, 85° F where the temperature swings five degrees. Monitoring temps near the thermostat reveals fairly close tolerance at the other side of the room you can have an unacceptable temperature swing. When you put a temperature datalogger in with your cultures (you do that, don’t you?) you find that the temperature control provided by your friendly room thermostat can swing three degrees or more depending on where in the room you measure. Temperature response is a core biological parameter and running off heedless and headless just gets you lost. But when you are experimenting with new strains, substrates, supplements, and techniques you better know how temperature affects results. Usually, you can incubate plates, flasks, jugs, and bags at room temperature with no incubator needed.
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